Simultaneous determination of jatrorrhizine, palmatine, berberine, and obacunone in Phellodendri Amurensis Cortex by RP-HPLC.
- Author:
Qian ZHANG
1
;
Lifen CAI
;
Guoyue ZHONG
;
Weizao LUO
Author Information
- Publication Type:Journal Article
- MeSH: Benzoxepins; analysis; Berberine; analogs & derivatives; analysis; Berberine Alkaloids; analysis; Chromatography, High Pressure Liquid; Drugs, Chinese Herbal; analysis; Limonins; analysis; Phellodendron; chemistry
- From: China Journal of Chinese Materia Medica 2010;35(16):2061-2064
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo develop a HPLC method to determine the content of jatrorrhizine, palmatine, berberine, and obacunone in Phellodendri Amurensis Cortex simultaneously. MEHTOD: The separations were carried out at 25 degrees C on a Phenomenex Gemini C18 column (4.6 mm x 250 mm, 5 microm) eluted with acetonitril and water containing 0.1% phosphoric acid in gradient mode. The flow rate was 1.0 mL x min(-1), detection wavelengthes were 345 nm for jatrorrhizine , palmatine, berberine and 210 nm for obacunone.
RESULTThe average recoveries of jatrorrhizine, palmatine, berberine, and obacunone were 98.94%, 101.17%, 96.22% and 98.90%, respectively.
CONCLUSIONThe method is simple, accurate and repeatable, and can be used in content determination of jatrorrhizine, palmatine, berberine, and obacunone in Phellodendri Amurensis Cortex.
