Determination of aurantio-obtusin and chrysophanol in cassiae semen by HPLC.

Author: Xiaoping HUANG ¹ ; Guoyue ZHONG ; Xuemei ZHANG ; Yi ZHANG
Author Information

1. Chongqing Academy of Chinese Materia Medica, Chongqing 400065, China.
Publication Type:Journal Article
MeSH: Anthraquinones; analysis; Chromatography, High Pressure Liquid; Cinnamomum aromaticum; chemistry; Drugs, Chinese Herbal; analysis; chemistry; Reproducibility of Results
From: China Journal of Chinese Materia Medica 2010;35(16):2065-2067
CountryChina
Language:Chinese
Abstract:
OBJECTIVETo improve the determination method of Cassiae Semen in China Pharmacopeia edition 2005. MMETHOD: Aurantio-obtusin and chrysophanol were separated on an Inertsil ODS-3 column with acetonitrile-0.1% phosphoric acid solutiona as mobile phase in gradient elution.
RESULTThe linear ranges of aurantio-obtusin and chrysophanol were 2.3-230, 2.87-286 mg x L(-1), respectively. The average recover were 100.3% (RSD 3.1%), and 99.5% (RSD 2.5%) respectively.
CONCLUSIONThis method is specific, accurate and reproducible, and can be used for the assay of Cassiae Semen in the new edition of Chinese pharmacopoeia.