Determination of chlorogenic acid and mangiferin in Folium Pyrrosiae from different habitats and species by HPLC.
- Author:
Kaitong LI
1
;
Yixuan ZHANG
;
Yang CAO
;
Yue SHI
Author Information
1. Institute of Medicinal Plant Development, Chinese Academy of Medical Sciences & Peking Union Medical College, Beijing 100193, China.
- Publication Type:Journal Article
- MeSH:
Chlorogenic Acid;
analysis;
Chromatography, High Pressure Liquid;
Plants, Medicinal;
chemistry;
Reproducibility of Results;
Xanthones;
analysis
- From:
China Journal of Chinese Materia Medica
2010;35(16):2075-2078
- CountryChina
- Language:Chinese
-
Abstract:
An HPLC method for simultaneous determination of chlorogenic acid and mangiferin in original medicinal materials and decoction pieces of Pyrrosiae Folium was developed. The assay was performed on a Diamonsil C18 (4.6 mm x 250 mm, 5 microm) column eluted with a mobile phase consisted of acetonitrile and 0.5% phosphoric acid solution in gradient elution at a flow rate of 1.0 mL x min(-1). The column temperature was set at 25 degrees C. The detection wavelength was 320 nm. The results showed that The linear ranges of chlorogenic acid and mangiferin were 5.2-130 mg x L(-1) (r = 0.9999) and 1.2-18 microg x mL(-1) (r = 0.9999), and the average recoveries (n=6) were 97.9% (RSD 1.9%) and 99.6% (RSD 2.9%), respectively. The method was simple, reproducible and valid. It can be used for quality evaluation and control of original medicinal materials and decoction pieces of Pyrrosiae Folium.
