Comparative studies on content of arbutin, bergenin and catechin in different part of Bergenia purpurascens and B. crassifolia.
- Author:
Xinguang SUN
1
;
Wenhua HUANG
;
Miao MA
;
Baolin GUO
;
Guiying WANG
Author Information
- Publication Type:Journal Article
- MeSH: Arbutin; analysis; Benzopyrans; analysis; Catechin; analysis; Chromatography, High Pressure Liquid; methods; Plant Components, Aerial; chemistry; Plant Roots; chemistry; Reproducibility of Results; Saxifragaceae; chemistry
- From: China Journal of Chinese Materia Medica 2010;35(16):2079-2082
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo develop a HPLC method for the determination of arbutin, bergenin and catechin in Chinese herb Bergenia, and to provide a scientific basis for evaluating the quality and reasonable utilization of the herb.
METHODThe HPLC analysis was achieved by using a C18 column and methanol-water as the mobile phase, with a flow rate of 1.0 mL min(-1), and detected by UV at 275 nm. The contents of arbutin, bergenin and catechin in the different parts of axial root, fibrous root and blade from Bergenia purpurascens and B. crassifolia.
RESULTThe contents of arbutin, bergenin and catechin have a few difference in B. purpurascens and B. crassifolia, and varies significantly in the different part of axial root, fibrous root and blade from some species. The contents of bergenin are higher in axial root, fibrous root, and the content of arbutin is higher in blade.
CONCLUSIONThis HPLC method can be used to determine simultaneously the content of arbutin, bergenin and catechin, and can establish a foundation for scientific study and evaluating the quality of species in Bergenia.
