Qualitative and quantitative analysis of plantamajoside in Plantaginis Herba.
- Author:
Qian SUN
1
;
Fang GENG
;
Xuemei CHENG
;
Li YANG
;
Zhengtao WANG
Author Information
1. Department of Pharmacognosy, China Pharmaceutical University, Nanjing 211198, China.
- Publication Type:Journal Article
- MeSH:
Asteraceae;
chemistry;
Catechols;
analysis;
Chromatography, High Pressure Liquid;
Drugs, Chinese Herbal;
chemistry;
Glucosides;
analysis;
Reproducibility of Results
- From:
China Journal of Chinese Materia Medica
2010;35(16):2095-2098
- CountryChina
- Language:Chinese
-
Abstract:
Plantamajoside is one of the main bioactive compounds in Plantaginis Herba A TLC method was developed identification of plantamajoside in 11 Plantaginis Herba samples using silica gel G as coating substance and a mixture of ethyl acetiate methanol-formic acid-water (18: 3 : 1.5 : 1) as a developing solvent, the established TLC condition displayed a very well separation on the chromatogram of tested Plantaginis Herba samples and the marker compound plantamajoside showed as a distinct light-blue fluorescence spot observed under UV 365 nm. Using the HPLC method, plantamajoside was separated at 30 degrees C on a Promocil C18, (4.6 mm x 250 mm, 5 microm) column with acetonitrile-0.1% formic acid (17:83) as the mobile phase. The detection wavelength was set at 330 nm and the flow rate was 1 mL x min(-1). The calibration curve of plantamajoside displayed ideal linearity over the range of 0.0499-11.9664 microg (r = 0.9999), and the average recovery of plantamajoside was 100.6% with a RSD of 2.7%. The contents of plantamajoside were in the range of 0.067%-1.80% in Plantaginis Herba The established TLC identification and HPLC were sensitive, reliable and repeatable, which can be applied for the quality evaluation and standard criteria of Plantaginis Herba.
