Quantitative determination of forsythiaside in Forsythia suspensa.
- Author:
Bohou XIA
1
;
Jingjing ZHU
;
Zhimin WANG
;
Limei LIN
;
Huimin GAO
Author Information
- Publication Type:Journal Article
- MeSH: Chromatography, High Pressure Liquid; Drugs, Chinese Herbal; analysis; Forsythia; chemistry; Glycosides; analysis; Reproducibility of Results
- From: China Journal of Chinese Materia Medica 2010;35(16):2110-2112
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo develop a quantitative determination of Forsythia suspensa and to provide scientific basis for its quality control.
METHODBy HPLC, the separation was performed on a Zorebax Eclipse XDB-C18 column (4.6 mm x 250 mm, 5 microm) at 25 degrees C using a isocratic elution of acetonitrile-water (containing 0.4% glacial acetic acid, 15:85). The detection wavelength was 330 nm.
RESULTThe calibration curve showed a good linearity (r = 0.9998) within test range of 0.202-1.515 microg. And the average recovery was 98.54% with RSD 1.1%. The content range of forsythoside A in 11 batches of Forsythiae Fructus was 0.200%-1.681%.
CONCLUSIONThe developed method is simple, accurate, specific, and with good repeatability. It is suitable for quality evaluation of Forsythiae Fructus.
