Assay of andrographolide bulk drug by HPLC.
- Author:
Wei XU
1
;
Xiuwei YANG
Author Information
- Publication Type:Journal Article
- MeSH: Chromatography, High Pressure Liquid; Diterpenes; analysis; Drugs, Chinese Herbal; analysis; Reproducibility of Results
- From: China Journal of Chinese Materia Medica 2010;35(16):2113-2115
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo establish a method for the determination of andrographolide bulk drug by HPLC.
METHODThe condition of HPLC was Diamonsil C18 (2) column (4.6 mm x 250 mm, 5 microm) with methanol-water (60:40) as a mobile phase. The flow rate was 1.0 mL x min(-1) and the detection wavelength was 235 nm.
RESULTThe standard curve was linear in the range of 0.5-5.0 microg and the correlation coefficient was 1.000. The average recoveries of the low, middle and high concentrations were 99.1%, 99.5% and 100.8%, respectively.
CONCLUSIONThe method is simple, precise and accurate, and it can be used for the quality control of andrographolide bulk drug.
