Simultaneous determination of multicomponent in Xuefuzhuyu granule with different UV-wavelength by HPLC.
- Author:
Zhenghui LIU
1
;
Tingxiang YE
;
Linlin ZHAO
;
Yi CHENG
;
Guoan LUO
Author Information
- Publication Type:Journal Article
- MeSH: Benzoates; analysis; Bridged-Ring Compounds; analysis; Chalcone; analogs & derivatives; analysis; Chromatography, High Pressure Liquid; methods; Coumaric Acids; analysis; Drugs, Chinese Herbal; analysis; chemistry; Glucosides; analysis; Monoterpenes; Quinones; analysis; Reproducibility of Results
- From: China Journal of Chinese Materia Medica 2010;35(16):2157-2160
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo establish the method for the quality control of Xuefuzhuyu granule.
METHODThe content of Semen Armeniacae Amarum PE, Hydroxysafflor yellow A, paeoniflorin and ferulic acid in Xuefuzhuyu granule were determined by HPLC under multi-wavelength of 210, 403, 230, 316 nm.
RESULTThe calibration curve of Semen Armeniacae Amarum PE. hydroxysafflor yellow A, paeoniflorin and ferulic acid were linear within 0.1154-0.9232 (r = 0.9998), 0.0219-0.1754 (r = 0.9994), 0.3952-3.1616 (r = 0.9995), and 0.0426-0.3408 microg (r = 0.9998), respectively. The average recoveries were 101.78% (RSD was 1.7%), 99.60% (RSD was 2.9%), 98.90% (RSD was 2.0%), and 100.31% (RSD was 1.8%), respectively.
CONCLUSIONThe method of quantification is accurate, rapid and reliable for the quality control of Xuefuzhuyu granule.
