Transmembrane transport and metabolism of diammonium glycyrrhizinate across rat small intestine in Ussing Chamber.
- Author:
Guofu LI
1
;
Jin YANG
;
Xiaoyi HUA
;
Rui CHEN
;
Haoru ZHAO
Author Information
- Publication Type:Journal Article
- MeSH: Animals; Biological Transport; Cell Membrane Permeability; Glycyrrhizic Acid; metabolism; pharmacokinetics; Hydrogen-Ion Concentration; Intestine, Small; chemistry; drug effects; metabolism; Male; Models, Biological; Rats; Rats, Sprague-Dawley
- From: China Journal of Chinese Materia Medica 2010;35(17):2261-2266
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo explore the transmembrane transport and metabolism of diammonium glycyrrhizinate in intestines of rats.
METHODAn Ussing Chamber model were used to investigate the transmembrane transport of diammonium glycyrrhizinate (GZ), the concentrations of diammonium glycyrrhizinate and its two metabolites were determined by HPLC.
RESULTThe permeability coefficients of GZ in difference intestinal mucous membranes were ranged from 0.3 x 10(-6) cm x s(-1) to 1.1 x 10(-6) cm x s(-1). The metabolism of GZ in enterocytes during its transport process was negligible. The concentration of diammonium glycyrrhizinate and pH had limit effects on the transport amount and the permeability coefficients of GZ.
CONCLUSIONGZ is a low permeability drug, but it can be absorbed at all segments of the small intestine in rats. Ileum is the major absorption region of GZ.
