OBJECTIVETo develop a HPLC method for the simultaneous determination of tectorigenin-7-O-xylosylglucosid, tectoridin and tectorigenin in flowers of Pueraria lobata.

METHODThe separation was performed on a Kromasil C18 column (4.6 mm x 250 mm, 5 microm), using a gradient elution with acetonitrile-water containing 0.2% phosphoric acid as the mobile phase. The flow rate was 1.0 mL x min(-1), the detection wavelength was 265 nm and the temperature of column was 35 degrees C.

RESULTThe linear ranges of tectorigenin-7-O-xylosylglucosid, tectoridin and tectorigenin were 0.14-1.40 (r = 0.9994), 0.20-2.02 (r = 0.9993), and 0. 10-1.04 (r = 0.9991) , respectively. The average recoveries (n=6) of the three constituents were 99.5% (RSD 1.8%), 99.6% (RSD 1.7%), and 99.8% (RSD 1.9%), respectively.

CONCLUSIONThe method is simple and accurate with a good eata and can be used as a quality control method for flowers of P. lobata of different sources.