Determination of ilexside II in leaves of Ilex cornuta by HPLC-ELSD.
- Author:
Zhimrong YAO
1
;
Jun LI
;
Mingbo ZHAO
;
Li WANG
;
Pengfei TU
Author Information
- Publication Type:Journal Article
- MeSH: Chromatography, High Pressure Liquid; methods; Drugs, Chinese Herbal; analysis; Glycosides; analysis; Ilex; chemistry; Plant Leaves; chemistry; Triterpenes; analysis
- From: China Journal of Chinese Materia Medica 2010;35(17):2312-2314
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo develop a HPLC-ELSD method for determination of ilexside II in leaves of Ilex cornuta.
METHODThe separation was performed on a Waters Syemmetry Shield RP18 column (4.6 mm x 250 mm, 5 microm) and methanol-water was used as mobile phase in gradient elution. The flow was 1.0 mL x min(-1), and the temperature was set at 35 degrees C.
RESULTThe calibration curve showed good linearity in the test range (R2 = 0.9997). And the mean recovery was 101.3%, RSD was 2.0% (n=6).
CONCLUSIONThe developed HPLC-ELSD method was accurate and reproducible, and can be used for the determination of ilexside II in leaves of Ilex cornuta.
