Determination of protopine in Corydalis racemose by HPLC.
- Author:
Xiazhi JIANG
1
;
Jinxia YE
;
Jianwei ZENG
;
Xiuhong ZOU
;
Jinzhong WU
Author Information
- Publication Type:Journal Article
- MeSH: Benzophenanthridines; analysis; Berberine Alkaloids; analysis; Chromatography, High Pressure Liquid; methods; Corydalis; chemistry; Drugs, Chinese Herbal; analysis
- From: China Journal of Chinese Materia Medica 2010;35(17):2315-2317
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo develop a HPLC method for determining the content of protopine in Corydalis racemose.
METHODAnalysis was performed on a Gemini C18 column (4.6 mm x 250 mm, 5 microm) eluted with acetonitrile-water containing 0.8% triethylamine and 3% acetic acid acetum (20:80) as the mobile phase. The flow rate was 1.0 mL x min(-1). The detection wavelength was 289 nm.
RESULTThe average content of protopine in Herb of Racemose Corydalis was 0.905%. The calibration curve of protopine was linear between 0.124-1.36 microg (r = 0.9999). The average recovery was 98.49% with RSD 1.9%.
CONCLUSIONThis method is simple, reproducible and can be used to determine the content of protopine in C. racemose.
