Effect of acupuncture serum on the number of osteclast cultured in vitro.
- Author:
Ying-xia ZHAO
1
;
Jing WANG
;
Yi-ren QIN
;
Yi WANG
;
Wei-rong XU
;
Xiao-hua TANG
;
Qi-ming YE
Author Information
- Publication Type:Journal Article
- MeSH: Acid Phosphatase; analysis; Acupuncture Points; Acupuncture Therapy; Animals; Animals, Newborn; Cell Count; Female; Humans; Osteoclasts; cytology; Osteoporosis, Postmenopausal; etiology; therapy; Rats; Rats, Sprague-Dawley
- From: Chinese Acupuncture & Moxibustion 2007;27(7):521-524
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo observe effects of acupuncture serum of ovariectomized rats on the number of in vitro cultural osteoclast of newborn rats.
METHODSForty 12-month-old SD female rats were randomly divided into 4 groups: sham-operation control group, ovariectomized (model) group, acupuncture prevention group and acupuncture treatment group. Osteoclasts isolated from long bone of the newborn SD rat were cultured on 24-well culture plates with DMEM containing 10% the test serum. Forty-eight hours later, the osteoclasts were stained with tartrate resistant acid phosphatase (TRAP) and the number of TRAP-positive multinucleated cells were counted.
RESULTSThe number of TRAP-positive multinucleated cells in the model group significantly increased as compared with the sham-operation control group (P < 0.01), and in the acupuncture prevention group and the acupuncture treatment group significantly declined compared with the model group (P < 0.05).
CONCLUSIONAcupuncture serum can equally reduce the number of osteoclasts in the two group.
