Effects of warming-needle therapy on gene expression pathways in the patient with knee osteoarthritis of deficiency-cold syndrome.
- Author:
Li-ping YANG
1
;
Ming-chen WANG
;
Wang-gen LIU
;
Mi-qu WANG
Author Information
- Publication Type:Journal Article
- MeSH: Acupuncture Therapy; methods; Aged; Female; Gene Expression Profiling; Gene Expression Regulation; Humans; Janus Kinases; genetics; MAP Kinase Signaling System; Male; Middle Aged; Myeloid Differentiation Factor 88; genetics; Needles; Osteoarthritis, Knee; metabolism; therapy; STAT Transcription Factors; genetics; Yang Deficiency; metabolism; therapy
- From: Chinese Acupuncture & Moxibustion 2007;27(9):677-680
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo explore the mechanism of warming-needle therapy in treatment of knee osteoarthritis of deficiency-cold syndrome.
METHODSEight cases of knee osteoarthritis of deficiency-cold syndrome were selected and treated with warming-needle therapy at Guanyuan (CV 4), Qihai (CV 6) , Zosanli (ST 36), etc.. The gene expression profiles before and after treatment in 4 cases who showed better therapeutic effect were compared. Taking ratio < 0.5 or ratio > 2.0 as differentially expression gene and obtaining differentially expression pathway (P < 0.5, n>3) by http://www. DAVID 2006.
RESULTSTwo cases were clinically cured, 4 cases were markedly effective, 1 case was effective and 1 case was ineffective. With help of the microarray, 449 differentially expression genes, and 10 differentially expression pathways were obtained including 2 energy metabolism pathways (oxidative phosphorylation, ATP synthetase), 4 cell signal transduction pathways (insulin signal pathway, Toll-like receptor signal pathway, JAK-STAT signal pathway, and MAPK signal pathway) and cell apoptosis pathway.
CONCLUSIONWarming-needle therapy is an effective therapy for knee osteoarthritis with deficiency-cold syndrome , which is possibly involved in the control and regulation of many gene expression by various signal transduction pathways.
