Comparison of conventional cytogenetics and interphase fluorescence in situ hybridization in diagnosis of acute promyelocytic leukemia.
- Author:
Rong WANG
1
;
Kou-Rong MIAO
;
Hai-Rong QIU
;
Si-Xuan QIAN
;
Ming HONG
;
Chun QIAO
;
Jian-Fu ZHAG
;
Lei FAN
;
Han-Xing WU
;
Hua LU
;
Hong-Xia QIU
;
Li-Juan CHEN
;
Shu-Jiang ZHAG
;
Wei XU
;
Peng LIU
;
Jian-Yong LI
Author Information
1. Department of Hematology, Nanjing Medical University, Jiangsu Provincial Hospital, Nanjing, Jiangsu Province, China.
- Publication Type:Journal Article
- MeSH:
Adolescent;
Adult;
Aged;
Aged, 80 and over;
Child;
Child, Preschool;
Cytogenetic Analysis;
methods;
Female;
Humans;
In Situ Hybridization, Fluorescence;
methods;
Infant;
Karyotyping;
Leukemia, Promyelocytic, Acute;
diagnosis;
genetics;
Male;
Middle Aged;
Young Adult
- From:
Journal of Experimental Hematology
2011;19(4):983-986
- CountryChina
- Language:Chinese
-
Abstract:
The aim of this study was to explore cytogenetic characteristics of acute promyelocytic leukemia (APL) and compare the interphase fluorescence in situ hybridization (I-FISH) with conventional cytogenetic (CC) analysis. A total number of 157 APL patients were recruited in this study, and the I-FISH and CC were applied to analyze cytogenetic features. Chromosome samples of bone marrow cells were prepared by short-term culture. Out of all 157 cases, 136 were observed with CC assay, 66 with I-FISH, of which 45 samples were analyzed with both methods. The results showed that among all 136 CC samples, t(15;17)(q22;q21) was found in 120 cases, of which 107 cases was isolated t(15;17)(q22;q21) abnormality, 13 cases was complex abnormalities and 12 case without mitotic figure. Among all 66 cases of I-FISH group, PMI/RARα fusion gene was found in 64 cases (97.0%), suggesting that I-FISH group was more sensitive than CC group (p = 0.041). It is concluded that combination of I-FISH and CC techniques plays a pivotal role for diagnosis and detection of minimal residual disease in APL.
