Effects of human aorta-gonad-mesonephros region stromal cells on inducing differentiation of murine embryonic stem cells into hematopoietic stem/progenitor cells in vitro.
- Author:
Yun CAI
1
;
Xun-Chao ZHANG
;
Hui-Qin CHEN
;
Bei-Yan WU
;
Shao-Liang HUANG
Author Information
1. Department of Pediatrics, SUN Yat-Sen University Third Affiliated Hospital, Guangdong Province, China.
- Publication Type:Journal Article
- MeSH:
Animals;
Cell Differentiation;
Cell Line;
Coculture Techniques;
Embryonic Stem Cells;
cytology;
Hematopoietic Stem Cells;
cytology;
Humans;
Mesonephros;
cytology;
Mice;
Stromal Cells;
cytology
- From:
Journal of Experimental Hematology
2011;19(4):1023-1027
- CountryChina
- Language:Chinese
-
Abstract:
This study was aimed to investigate the effect of human aorta-gonad-mesonephros (AGM) region stromal cells on differentiation of murine embryonic stem cells (ESC) into hematopoietic stem cells (HSC) in vitro and to clarify their effect mechanism. E14 murine ESC were induced into embryo body (EB) firstly. Then the EB cells were further co-cultured with the stromal cells from human AGM region, fetal liver (FL) or bone marrow (BM) in Transwell non-contact system. According to the different culture methods, the EB cells were divided into 6 groups including EB control group, AGM group, FL group, BM group, AGM + FL group and AGM + BM group. The induced cells derived from EB were collected for Sca-1(+)/c-Kit(+) cells analysis by flow cytometry and colony forming unit (CFU) assay. The results showed that Sca-1(+)/c-Kit(+) cell proportion of EB cells significantly increased after being induced by different stromal cells (p < 0.01). The AGM + FL group had most Sca-1(+)/c-Kit(+) cells for the positive cell proportion reached (21.96 ± 2.54) % (p < 0.01). The Sca-1(+)/c-Kit(+) cell proportion of AGM + BM group was much high than that of BM group too (p < 0.01). The EB control group showed CFU amount less than any other groups, while the CFU amount of AGM + FL, AGM + BM groups were higher, especially in the AGM + FL group (p < 0.01). It is concluded that the human AGM region stromal cells may help to maintain certain number of primitive HSC with high proliferation potential. Human AGM region, FL or BM stromal cells, applied in sequential orders, can significantly expand in vitro the primitive hematopoietic stem/progenitor cells derived from ESC.
