Clinical value of interphase fluorescent in situ hybridization in diagnosis of core-binding factor acute myelocytic leukemia.
- Author:
Hui YANG
1
;
Lei FAN
;
Hai-Rong QIU
;
Rong WANG
;
Jian-Fu ZHANG
;
Yu-Jie WU
;
Jian-Yong LI
;
Peng LIU
Author Information
1. Department of Hematology, Jiangsu Province Hospital, Nanjing, Jiangsu Province, China.
- Publication Type:Journal Article
- MeSH:
Adolescent;
Adult;
Child;
Core Binding Factors;
genetics;
Cytogenetic Analysis;
Female;
Humans;
In Situ Hybridization, Fluorescence;
methods;
Karyotyping;
Leukemia, Myeloid, Acute;
diagnosis;
genetics;
Male;
Middle Aged;
Young Adult
- From:
Journal of Experimental Hematology
2011;19(5):1156-1160
- CountryChina
- Language:Chinese
-
Abstract:
The purpose of this study was to evaluate the clinical value of interphase fluorescence in situ hybridization (I-FISH) in diagnosis of core-binding factor acute myelocytic leukemia (CBF AML). The cytogenetic characteristics in leukemia cells from 82 cases of AML-M(2) and 43 cases of AML-M(4)/M(5) were detected by using I-FISH with AML1-ETO double color double fusion probe and double color break point isolated gene probe CBFβ-MYH11, and the detected results were compared with results detected by conventional cytogenetic R banding technique (CC). The results indicated that AML1-ETO fusion gene was detected in 30.5% cases (25/82) by FISH, and t(8;21)(q22;q22) karyotypic aberrations was found in 28.0% cases (23/82) by CC method. Among 25 FISH positive cases, typical FISH positive signal pattern (1R1G2F) was displayed in 22 cases and atypical signal pattern (1R2G1F and 2R1G2F) was found in the other 3 cases. Among all 43 AML-M(4)/M(5) cases, the CBFβ-MYH11 fusion gene was detected in 23.3% cases (10/43) by FISH, which sensitivity was significant higher than that by CC method (2/43) (p < 0.05). It is concluded that some insufficiency of CC technique can be compensated by FISH, and combination of I-FISH with CC technique play a crucial role in diagnosis of CBF AML and in monitoring of minimal residual disease.
