Expression of DNA-dependent protein kinase catalytic subunit in adult acute leukemia and its significance.
- Author:
Rui SHEN
1
;
Xie-Lan ZHAO
;
Zhao OUYANG
;
Min-Yuan PENG
;
Jian ZHANG
;
Jia-Jia LIANG
Author Information
1. Department of Hematology, Central South University, Changsha, Hunan Province, China.
- Publication Type:Journal Article
- MeSH:
Adolescent;
Adult;
Aged;
Aged, 80 and over;
Chromosome Aberrations;
DNA-Activated Protein Kinase;
genetics;
metabolism;
Female;
Humans;
Karyotype;
Leukemia;
diagnosis;
genetics;
metabolism;
Male;
Middle Aged;
Nuclear Proteins;
genetics;
metabolism;
Prognosis;
Young Adult
- From:
Journal of Experimental Hematology
2011;19(5):1180-1183
- CountryChina
- Language:Chinese
-
Abstract:
This study was aimed to explore the expression of DNA-dependent protein kinase catalytic subunit (DNA-PKcs) in adult acute leukemia and its correlation with clinical characteristics, karyotype and prognosis. Indirect immunofluorescent cytometry was used to detect the expression of DNA-PKcs in bone marrow mononuclear cells of 105 patients with acute leukemia before chemotherapy and 41 of them after 2 cycles of chemotherapy. Cytogenetic data were obtained from 26 of them by R band karyotypic analysis. The results showed that the expression of DNA-PKcs was correlated with higher WBC count level in peripheral blood (p < 0.05), but was not obviously associated with median age, gender, percentage of bone marrow blasts, clinical classification, median hemoglobin level and median platelet count (p > 0.05). The middle and strong positive expression of DNA-Pkcs in non-remission group was significantly higher than that in remission group (p < 0.05). The positive rate of DNA-PKcs in abnormal chromosome group was significantly higher than that in chromosome normal group (p < 0.05). It is concluded that the DNA-PKcs expression level is closely related with the increased WBC count, and the expression of DNA-PKcs is correlated also with karyotype and clinical prognosis in adult acute leukemia.
