Biological effects of low dose X-irradiation on human bone marrow mesenchymal stem cells.
- Author:
Bing WU
1
;
Yan WEI
;
Feng-Qiang LIU
;
Qian ZHANG
;
Cun-Bang WANG
;
Hai BAI
Author Information
1. Institute of Hematology, Lanzhou Military Area General Hospital, Lanzhou, Gansu Province, China.
- Publication Type:Journal Article
- MeSH:
Bone Marrow Cells;
radiation effects;
Cell Differentiation;
Cell Proliferation;
Cells, Cultured;
Dose-Response Relationship, Radiation;
Humans;
Mesenchymal Stromal Cells;
radiation effects;
X-Rays
- From:
Journal of Experimental Hematology
2011;19(5):1214-1217
- CountryChina
- Language:Chinese
-
Abstract:
Recent studies have shown that low dose X-irradiation shows specific effect different from high dose exposures. However, the biologic responses of bone marrow mesenchymal stem cells (BM-MSC) to low dose X-irradiation have rarely been described in the literature. This study was purposed to investigate the biologic responses of human bone marrow-derived MSC to low dose X-irradiation. The proliferation of cells was tested with MTT assay, so that the cell growth curve was drawn at 1 to 7 days. The expression of survivin mRNA was detected by RT-PCR assay; the BM-MSC DNA damage induced by X-irradiation were detected with mononuclear cell gel electrophoresis. The results indicated that the proliferative ability of BM-MSC exposed to low doses of X rays was obviously enhanced as compared with control group. The low dose X-irradiation caused the damage of DNA in X-ray dose dependent manner. X-irradiation enhanced expression of survivin in MSC. It is concluded that the low dose below 20 cGy of X-irradiation has a promoting effect on survivin expression in BM-MSC. Whether the high expression of survivin plays an important role to resist ionizing radiation needs to be further studied.
