Analysis of immunophenotype characteristics in 109 cases of B lineage chronic B lymphoid leukemia.
- Author:
Ai-qing WANG
1
;
Mei-Ju GENG
;
Ming-qing ZHU
;
Li CHEN
Author Information
1. Soochow University Experiment Center, Suzhou, Jiangsu Province, China.
- Publication Type:Journal Article
- MeSH:
Adult;
Aged;
Aged, 80 and over;
Female;
Flow Cytometry;
Humans;
Immunophenotyping;
Leukemia, Lymphocytic, Chronic, B-Cell;
diagnosis;
immunology;
Male;
Middle Aged;
Neoplasm, Residual;
diagnosis;
immunology;
Prognosis
- From:
Journal of Experimental Hematology
2011;19(6):1374-1377
- CountryChina
- Language:Chinese
-
Abstract:
This study was aimed to investigate the immunophenotypic characteristics of 109 cases of B-cell chronic lymphoid leukemia (B-CLL) so as to provide evidences for the diagnosis and therapy of B-CLL, and for the detection of the minimal residual disease and its prognosis. Immunophenotyping was performed in 109 patients of B-CLL by two/three color multiparameter flow cytometry analysis using a panel of monoclonal antibodies. The results showed that in 109 cases of B-CLL, all cases expressed CD19, the positive ratios of other B lineage antigen such as CD20, CD22 and CD23 were 95.40%, 94.50%, 86.20% respectively. None of the B-CLL cases expressed CD10. The expression ratio of FMC-7 and CD38 in 105 cases of B-CLL were 28.60% and 36.20%. Among the B-CLL cases the CD5(+) cells amounted to 86.23%, CD5(-) cells amounted to 13.76%, ZAP-70 protein was expressed in 12 out of 50 patients. It is concluded that immunophenotypic data are very useful for the diagnosis and detection of minimal residual disease of B-CLL, and the relationship between the immunophenotypic characteristics and the prognosis of B-CLL needs further study.
