Establishment of experimental angiogenic models with applications of quantitative digital image analysis.
- Author:
Jin-ling JIANG
1
;
Wei-ren LIU
;
Ying-yan YU
;
Pei-hua NI
;
Jian-lin WU
;
Jun JI
;
Jia-nian ZHANG
;
Xue-hua CHEN
;
Bing-ya LU
;
Zheng-gang ZHU
Author Information
- Publication Type:Journal Article
- MeSH: Animals; Cell Line, Tumor; Cells, Cultured; Chorioallantoic Membrane; blood supply; Diagnostic Imaging; methods; Homeodomain Proteins; genetics; metabolism; physiology; Human Umbilical Vein Endothelial Cells; Humans; Neovascularization, Pathologic; Neovascularization, Physiologic; Software; Stomach Neoplasms; genetics; metabolism; pathology; Transcription Factors; genetics; metabolism; physiology; Transfection
- From: Chinese Journal of Pathology 2011;40(7):475-479
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo establish experimental models for tumor neovascularization and to apply quantitative digital imaging analysis in the study.
METHODSAn endothelial tube formation model was established by human umbilical vein endothelial cells (HUVECs). A vasculogenic mimicry model was established by SGC-7901 gastric cancer cell line. Fertilized eggs were used to establish a chorioallantoic membrane angiogenesis model. Using gene transfection experiment, IRX1 tumor suppressor gene was chosen as a therapeutic target. Image Pro Plus (IPP) analysis software was used for digital vascular images analysis with parameters including points, lines, angles and integral absorbance (IA) for the tubular formation or vasculogenic mimicry.
RESULTSDigital image analysis by IPP showed that HUVEC tubular formation was significantly inhibited in IRX1 transfectant, compared with controls. The tubular numbers in three groups were 12.80 +/- 3.83, 29.00 +/- 5.34 and 28.20 +/- 4.32 (P<0.01). The connection points of tubules in three groups were 13.20 +/- 2.59, 25.00 +/- 2.24 and 24.60 +/- 3.21 (P<0.01). The tubular lengths of three groups were (821.5 +/- 12.5), (930.9 +/- 13.5) and (948.4 +/- 18.1) microm (P=0.022). The IA values of PAS stain in three groups were 3606 +/- 363, 14 200 +/- 1251 and 15 043 +/- 1220 (P<0.01). In chick chorioallantoic membrane model, the angular numbers of tubules in three groups were 6.41 +/- 2.60, 10.27 +/- 2.65 and 9.18 +/- 1.99 (P<0.01).
CONCLUSIONSThe endothelial tube formation model, vasculogenic mimicry model and chorioallantoic membrane angiogenesis model are useful for gene therapy and drug screening with targeting neoplastic vascularization. Professional image analysis software may greatly facilitate the quantitative analysis of tumor neovascularization.
