Molecular typing of Salmonella paratyphi A isolates from four provinces with pulse-field gel electrophoresis.
- Author:
Wei LI
1
;
Zhi-gang CUI
;
Zheng ZHANG
;
Ke-cheng TIAN
;
Hao-jie ZHONG
;
Feng-cai ZHU
;
Wei-li LIANG
;
Li-juan ZHANG
;
Biao KAN
Author Information
- Publication Type:Journal Article
- MeSH: China; Electrophoresis, Gel, Pulsed-Field; Salmonella paratyphi A; classification; genetics; isolation & purification
- From: Chinese Journal of Epidemiology 2006;27(10):871-874
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo analyze the molecular types of Salmonella paratyphi A strains isolated in the recent years, and to construct the standard S. paratyphi A databank in the laboratory surveillance network PulseNet China.
METHODSS. paratyphi A isolates from 4 provinces were analyzed with the standard pulsed-field gel electrophoresis (PFGE) protocol used in PulseNet and their patterns compared. The databank was constructed with BioNumerics.
RESULTSEleven PFGE patterns were obtained, in which 3 predominant patterns were identifies with a similarity coefficient of 96.3%. The strains of these patterns, accounted for 86.5% of the analyzed strains, appeared in different provinces and years.
CONCLUSIONThe databank of S. paratyphi A was constructed and could be used in laboratory surveillance of S. paratyphi A in PulseNet China. From the analyses on molecular typing of the isolates, data suggested that the predominant strains might cause the epidemics in different regions.
