Study on the characteristics of mutation on Mycobacterium tuberculosis rifampicin-resistance gene.
- Author:
Jing-hua LIU
1
;
Li-shui ZHANG
;
Zhi-guang LIU
;
Ming-xiang HUANG
;
Xiu-qin ZHAO
;
Lin WANG
;
Kang-lin WAN
Author Information
- Publication Type:Journal Article
- MeSH: Antibiotics, Antitubercular; pharmacology; Bacterial Proteins; genetics; DNA Mutational Analysis; DNA-Directed RNA Polymerases; Drug Resistance, Bacterial; Mycobacterium tuberculosis; drug effects; genetics; Polymerase Chain Reaction; Polymorphism, Single-Stranded Conformational; Rifampin; pharmacology
- From:Chinese Journal of Epidemiology 2006;27(11):973-976
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo elucidate the characters of rpoB mutation in rifampin-resistant clinical isolates of Mycobacterium tuberculosis.
METHODS286 bp DNA fragment of rpoB gene including 81 bp code region (rifampin resistance deteremination region, RRDR) was analyzed by PCR-single-strand conformation polymorphism(SSCP). The 286 bp DNA fragment of each strain which had been proved to have mutation by PCR-SSCP was then sequenced. 110 strains of M. tuberculosis, including 73 rifampin-resistant strains, 11 rifampin-susceptible drug-resistant strains and 26 drug-susceptible strains were studied.
RESULTS47 rifampin-resistant strains were detected to have mutations by PCR-SSCP method. 76.6% rifampin-resistant strains showed that rpoB gene was carrying single point mutation analyzed by direct sequencing technique, which mainly located at 531-Ser (61.1%) and 526-His (25.0%). The combined mutation rate was 23.4%. In addition, 2 rifampin-susceptible drug-resistant strains and 1 drug-susceptible strain were mutated, detected by PCR-SSCP method. Sequencing results showed that the mutations located at 511-Leu, 526-His and 535-Pro.
CONCLUSIONMutations in the 81 bp RRDR of rpoB were the main reasons of M. tuberculosis resistant to rifampin. 531-Ser and 526-His were the most common positions of mutations.
