Study on HIV-1 related genetics and threshold on drug resistance in Dehong prefecture of Yunnan province in 2012.

Min Chen; Jibao Wang; Hui Xing; Yanling MA; Shitang Yao; Huichao Chen; Jin Yang; Yanling LI; Song Duan; Manhong Jia

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Study on HIV-1 related genetics and threshold on drug resistance in Dehong prefecture of Yunnan province in 2012.

Author: Min CHEN ¹ ; Jibao WANG ² ; Hui XING ³ ; Yanling MA ¹ ; Shitang YAO ² ; Huichao CHEN ¹ ; Jin YANG ² ; Yanling LI ² ; Song DUAN ⁴ ; Manhong JIA ⁵
Author Information

1. Yunnan Provincial Center for Disease Control and Prevention, Kunming 650022, China.
2. Dehong Center for Disease Control and Prevention.
3. National Center for AIDS/STD Control and Prevention, Chinese Center for Disease Control and Prevention.
4. Dehong Center for Disease Control and Prevention. Email: dhduansong@sina.com.
5. Yunnan Provincial Center for Disease Control and Prevention, Kunming 650022, China. Email: jiamanhong@hotmail.com.
Publication Type:Journal Article
MeSH: Adolescent; Adult; China; epidemiology; Drug Resistance, Viral; genetics; Female; Genotype; HIV Infections; epidemiology; virology; HIV-1; drug effects; genetics; Humans; Male; Young Adult
From: Chinese Journal of Epidemiology 2014;35(8):909-912
CountryChina
Language:Chinese
Abstract:
OBJECTIVETo study the HIV-1 genotypes and transmitted drug resistance (TDR) in Dehong prefecture of Yunnan province in 2012.
METHODSReferring to the guidelines for HIV drug resistance threshold survey (HIVDR-TS), 60 plasma samples of recently reported HIV-infected individuals between 16 and 25 years old were collected in Dehong prefecture from January to August 2012. Genotyping of partial pol gene was performed by using reverse transcriptional PCR. HIV-1 genotype and the prevalent levels of HIV-1 drug resistance transmission were analyzed.
RESULTS52 plasma samples were successfully sequenced and analyzed. Among them, 59.6% were Chinese, and the rest (40.4%) were Burmese. Based on pol sequences, identified HIV genotypes would include unique recombinant forms (URFs, 38.5%), subtype C (34.6%), CRF01_AE (21.2%), CRF08_BC (3.8%), and subtype B (1.9%). One drug resistant mutation to non-nucleoside reverse transcriptase inhibitors (NNRTIs) was detected in respective two sequences. Based on the statistical method of HIVDR-TS, the prevalence of transmitted HIV-1 drug resistance was adjusted as a moderate level (5%-15%).
CONCLUSIONDiverse HIV-1 genotypes were found in this study, and the current HIV-1 drug resistant strains transmission was catalogued as moderate prevalence level in Dehong.