Relationship between cholesteryl ester transfer protein gene-629C→A mutations with HDL-C levels and coronary heart disease
10.3760/cma.j.issn.0253-3758.2013.05.010
- VernacularTitle:胆固醇酯转运蛋白-629C→A基因变异与血清高密度脂蛋白胆固醇水平和冠心病的关系
- Keywords:
Coronary disease;
Nucleoside transfer proteins;
Polymorphism,sigle nucleotide
- From:
Chinese Journal of Cardiology
2013;41(5):399-405
- CountryChina
- Language:Chinese
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Abstract:
Objective To investigate the relationship between the-629C/A polymorphism in the promoter region of the CETP gene,serum Levels,lipid metabolism,and coronary heart disease (CHD) among Tianjin Han Chinese population.Methods A hospital-based case-control study was conducted in Tianjin Chest Hospital from 2010 October to 2011 October.The subjects underwent angiography were divided into a case group (n =429) and a control group (n =275).The CETP gene promoter polymorphism at position-629 was determined by restricted fragment length polymorphism using the polymerase chain reaction (PCR-RFLP) method.The serum CETP levels was determined by enzyme-linked immunosorbent assay (ELISA) method.Results (1) The frequencies of-629A allele in Tianjin Han Chinese population was 0.408,significantly lowever than that in other domestic and foreign populations (0.479-0.701,P ＜ 0.05).(2) Variant AA genotype showed reduced CETP levels (P ＞ 0.05) and higher HDL-C levels (P ＜0.05),compared to wild CC genotype.(3) Although there was a negative trend correlation between serum CETP and HDL-C levels,it did not reach statistical significance (P ＞ 0.05).(4) There were significant differences in the frequencies of CETP gene-629 genotype and allele between the two groups (P ＜ 0.001),carries with CA/AA genotype and A allele showed higher risk of CHD,OR (95％ CI) values were 4.627 (3.163-6.769) 、8.779 (4.799-16.059) and 3.173 (2.453-4.104) respectively.There was no relationship between CETP-629C/A polymorphism and coronary artery stenosis degree (x2 =3.588,P =0.166).Conclusion The frequencies of CETP gene-629 genotype and allele in the Tianjin Han Chinese population was significantly different from that in Other domestic and foreign populations.Variant AA genotype,which showed reduced CETP levels and higher HDL-C levels,is paradoxically associated with increased risk of CHD.Thus,CETP gene variation may affect coronary risk apart from the level of HDL-C.
