Impact and potential mechanism of human α-defensin 1 on low-density lipoprotein oxidation ability of ECV304 cells
10.3760/cma.j.issn.0253-3758.2013.05.011
- VernacularTitle:人α-防御素对人内皮细胞ECV304氧化低密度脂蛋白能力的影响及其机制的实验研究
- Author:
Cheng-Jie XU
1
;
Jing-Wei WANG
;
Jian SONG
;
Jun-Zhu WU
;
Ting-Ting SONG
Author Information
1. 430071,武汉大学基础医学院生物化学与分子生物学系
- Keywords:
Atherosclerosis;
Endothelial cells;
Alpha-defensins;
Reactive oxygen species;
Lipoproteins,LDL
- From:
Chinese Journal of Cardiology
2013;41(5):406-410
- CountryChina
- Language:Chinese
-
Abstract:
Objective To explore the role and potential mechanism of human α-defensin 1 (HNP-1) on low-density lipoprotein (LDL) oxidation ability of human endothenial cells (EVC304).Methods Post incubation with LDL for 3 h,the malondiadehyde (MDA) and protein carbonyl (PCO) were detected in untreated ECV304 (control) and in HNP-1 transfected ECV304 in the presence and absence of siRNA against HNP-1.Flow cytometry and fluorescence microscopy were used to detect the generation of oxygen free radical in the ECV304 which have been pretreated by LDL,LPS and HNP-1,respectively.Result Compared with control group,MDA level was significantly increased in HNP-1 transfected[(4.21 ± 0.03) vs.(3.15 ± 0.02) nmol/mg · pro] or in HNP-1stimulated ECV304 cells [(14.49 ± 1.10) vs.(9.47 ± 1.18) nmol/mg · pro],which could be significantly downregulated by siRNA [(3.76 ± 0.48) vs.(4.54 ± 0.28) nmol/mg · pro,all P ＜ 0.05].PCO was also significantly increased in HNP-1 transfected ECV304 cells.The levels of free radical were significantly increased in HNP-1 transfected or HNP-1 stimulated ECV304 cells.Conclusion HNP-1 can enhance the LDL oxidation ability of human endothelial cells via promoting the generation of free radicals.
