Role of the Th17/Treg functional imbalance on the development of atherosclerosis in apo E knockout mice
10.3760/cma.j.issn.0253-3758.2013.05.013
- VernacularTitle:动脉粥样硬化小鼠辅助性T细胞17和调节性T细胞功能失衡的机制研究
- Keywords:
Atherosclerosis;
T-lymphocytes,helper inducer;
T-lymphocytes,regulatory
- From:
Chinese Journal of Cardiology
2013;41(5):416-421
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the role of the helper T cells (Th) 17/Treg cell imbalance on the development of atherogenesis in apo E knockout mice.Methods Apo E-/-mice were examined at age of 6,12,24 and 48 weeks (n =10 each).Age matched C57/B6 mice served as controls.The number of Th17,Treg and dendritic cell (DC) was detected by flowcytometry.The levels of interleukin(IL)-6,IL-17A and transforming growth factor(TGF)-β1 were detected by ELISA.The suppression ability of Treg was evaluated by mixed lymphocyte reaction.Results With increasing ages,the frequencies of Th17 and Treg in CD4+ T cells were increased(Tb17 ratio from 1.00％ to 3.14％ ; Treg ratio from 8.08％ to 27.80％) and the level of IL-17A was up-regulated [from (87 ± 15) pg/ml to (191 ±26) pg/ml],but the rate of Th17/ Treg cell and the level of TGF-β1 remained stable during atherogenesis in apo E knockout mice.Furthermore,the phenotype of splenic DC was matured and the blood level of IL-6 was up-regulated [from (43 ±5) pg/ml to (104 ±11) pg/ml] with aging in apo E / mice.Addition of IL-6 to T cells reversed the ability of Treg to suppress the proliferation of effective T cells.Conclusion DC overactivation,subsequent increased secretion of IL-6,inhibition of Treg cell function and the Th17/Treg cell imbalance play key roles on the atherogenesis in apo E-/-mice.
