Pretreatment with cyclosporin A nanoparticles emulsion protects apoptosis of swine adipose tissue-derived stem cells
10.3760/cma.j.issn.0253-3758.2013.06.014
- VernacularTitle:环孢菌素A-纳米乳预处理抑制猪脂肪组织来源干细胞的凋亡
- Author:
Qiao-Xiang YIN
1
;
Zhi-Yong PEI
;
Heng WANG
;
Yu-Sheng ZHAO
Author Information
1. 空军总医院干部病房心内科
- Keywords:
Stem cells;
Cyclosporine;
Apoptosis;
Nanoparticles
- From:
Chinese Journal of Cardiology
2013;41(6):501-506
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the effect of cyclosporine A-nanopaticals emulsion (CsA-NP) on protecting apoptosis of swine adipose tissue-derived stem cells (ASC) and related mechanisms.Methods ASC were randomized to six groups:contronl group,single H2O2 group,CsA or CsA-NP 0.1 mg/ml + H2O2 group,CsA or CsA-NP 1.0 mg/ml + H2O2 group,CsA or CsA-NP 5.0 mg/ml + H2O2 group,CsA or CsA-NP 10.0 mg/ml + H2O2 group.ASC apoptosis was induced by hydrogen peroxide (H2O2 100 μ mol/L) in vitro.The morphology of apoptotic cells was observed and the number of apoptotic cells was measured.Apoptosis of ASC was detected by flow cytometry using an apoptosis kit.Cell activity was determined by CCK-8 assay.Caspase-3 activity was detected by applying a caspase-3 assay kit.Expression of cytochrome C was investigated by Western blot.Results H2O2 induced ASC apoptosis was evidenced by morphological and biochemical changes,which could be significantly reduced by pre-treatment with CsA or CsA-NP at concentration of 0.1-10.0 mg/ml,and the best effect was observed at concentration of 5 mg/ml (apoptosis rate:CsA:10.6％ ±2.8％ vs.25.2％ ±3.8％; CsA-NP:6.2％ ±2.6％ vs.25.2％ ±3.6％ in control group,all P ＜ 0.01).The cell activity was significantly higher in CsA or CsA-NP pre-treated ASC at concentration of 0.1-10.0 mg/ml than in H2O2 group (P ＜0.01).Pre-treatment with CsA or CsA-NP (0.1-10.0 mg/ml) significantly down-regulated caspase-3 activity.Furthermore,CsA or CsA-NP (5 mg/ ml) completely inhibited the H2O2-induced release of cytochrome C.Conclusions These results suggest that CsA-NP and CsA could protect the oxidative stress-induced ASC apoptosis through decreasing the activation of caspase-3 and inhibiting the release of cytochrome C.
