Study on gene expression changes of arsenic sulfide treated NB4 cells.
- Author:
Chunhong GU
1
;
Fangyuan CHEN
;
Jieying HAN
;
Ye TENG
;
Nianxian SHAO
;
Renrong OUYANG
Author Information
- Publication Type:Journal Article
- MeSH: Arsenicals; pharmacology; Gene Expression Regulation, Neoplastic; drug effects; Humans; Oligonucleotide Array Sequence Analysis; Sulfides; pharmacology; Tumor Cells, Cultured; drug effects; metabolism
- From: Chinese Journal of Hematology 2002;23(1):16-18
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo compare the gene expression status of NB4 cells before and after arsenic sulfide treatment by cDNA microarray.
METHODSTwo cDNA probes were made from mRNA of untreated or arsenic sulfide treated NB4 cells. The cells were labelled with Cy3 or Cy5 fluorescence dyes individually, hybridized with cDNA microarray, and scanned for fluorescent intensity. The altered gene expression was screened through the analysis of difference in gene expression profile.
RESULTSThirty four genes related to apoptosis, cell cycle and others expressed different after the treatment of arsenic sulfide, 28/34 were up-regulated, 6/34 down-regulated.
CONCLUSIONABC50, PNAS-2 and cyclin G(2) might take part in the process of NB4 cell apoptosis induced by arsenic sulfide.
