Expression and clinical significance of anti-apoptosis gene, survivin, in acute leukemia.
- Author:
Maofang LIN
1
;
Xiaoli MENG
;
Zhen CAI
;
Xiujin YE
Author Information
- Publication Type:Journal Article
- MeSH: Adolescent; Adult; Aged; Antineoplastic Agents; therapeutic use; Chromosomal Proteins, Non-Histone; genetics; metabolism; Drug Resistance, Neoplasm; genetics; physiology; Female; Gene Expression Regulation, Leukemic; Humans; Inhibitor of Apoptosis Proteins; Leukemia, Myeloid, Acute; drug therapy; genetics; metabolism; Male; Microtubule-Associated Proteins; Middle Aged; Neoplasm Proteins; Precursor Cell Lymphoblastic Leukemia-Lymphoma; genetics; metabolism; RNA, Messenger; biosynthesis; Remission Induction; Treatment Outcome
- From: Chinese Journal of Hematology 2002;23(5):251-253
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo explore the correlation between expression of surviving gene in acute leukemic cells and its clinical effects.
METHODSBy using semi-quantitative reverse transcriptase polymerase chain reaction (RT-PCR) technique, surviving gene expression in 50 previously untreated acute leukemia (AL) patients was analysed. The apoptosis of primary leukemia cells cultured in vitro was assayed with terminal deoxyribonucleotidyl transferase mediated dUTP-biotin nick end labeling (TUNEL).
RESULTSSurviving gene expression levels in cells of AL patients at diagnosis were significantly higher than that in normal bone marrow mononuclear cells (MNCs) (82.0% vs 33.3%, P < 0.05). The expression level was higher in ALL cells than in ANLL cells (89.5% vs 75.0%). Among 22 cases of ANLL, bone marrow remission (BMR) rate was higher in surviving gene negative expression cells from patients accepted a course of chemotherapy than in positive expression cells (83.3% vs 25.0%, P = 0.023). Among 13 ANLL patients received a course of HA regimen chemotherapy, the BMR was higher in patients surviving mRNA negative expression cells than in positive cells (100.0% vs 27.3%). Patients with surviving/beta-actin ratio>0.6 attained lower BMR.
CONCLUSIONHigher expression level of surviving mRNA in AL cells may be one of the reasons that leukemic cells are insensitive to chemotherapy.
