The expression of human telomerase-associated protein hTERT and TEP1 in cord blood stem/progenitor cells and its significance.
- Author:
Yanping MA
1
;
Ping ZOU
;
Juan XIAO
;
Shiang HUANG
Author Information
- Publication Type:Journal Article
- MeSH: Antigens, CD34; immunology; Carrier Proteins; genetics; DNA-Binding Proteins; Fetal Blood; cytology; metabolism; Gene Expression; Humans; Leukocytes, Mononuclear; cytology; immunology; metabolism; RNA, Messenger; genetics; metabolism; Reverse Transcriptase Polymerase Chain Reaction; Stem Cells; cytology; metabolism; Telomerase; genetics
- From: Chinese Journal of Hematology 2002;23(4):183-186
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo explore the regulatory effects of hTERT and TEP1 on telomerase activity in hematopoiesis.
METHODSThe hTERT and TEP1 mRNA expression was detected by RT-PCR and the telomerase activi-ty by TRAP.
RESULTSIn mononuclear cells (MNC) and CD(34)(-) cells, no detectable telomerase activity and hTERT mRNA expression were found. CD(34)(+) cells showed hTERT expression and a low level telomerase activity. TEP1 mRNA was detected in MNC, CD(34)(-) and CD(34)(+) cells with no significant difference in the expression level. In the CD(34)(+) cells cultured in vitro with growth factors for 7 days, the telomerase activity and the expression of hTERT mRNA were upregulated, but were downregulated in the long time culture. No significant changes in TEP1 expression was observed.
CONCLUSIONIn the course of hematopoiesis, hTERT mRNA expression was in accordance with telomerase activity, hTERT gene plays a crucial role in the expression of telomerase activity, while TEP1 gene plays, if any, a much smaller role.
