Culture and pluripotentiality of human marrow mesenchymal stem cells.

Author: Wenyu FU ¹ ; Yanmeng LU ; Yingjie PIAO
Author Information

1. Central Laboratory, The First Military Medical University, Guangzhou 510515, China.
Publication Type:Journal Article
MeSH: Animals; Bone Marrow Cells; cytology; Cell Culture Techniques; methods; Cell Differentiation; Cell Division; Cell Transplantation; methods; Cells, Cultured; Humans; Mesoderm; cytology; Mice; Mice, Nude; Stem Cells; cytology; Transplantation, Heterologous
From: Chinese Journal of Hematology 2002;23(4):202-204
CountryChina
Language:Chinese
Abstract:
OBJECTIVETo cultivate human mesenchymal stem cells (MSC) derived from fetal bone marrow and examine their pluripotentiality.
METHODSBone marrow mononuclear cells from human fetal femur were collected by Percoll gradient centrifugation. The low density cells including MSCs were cultivated and expanded in MSCGM media. The characteristics of the multipotent MSCs were observed by implanting them into nude mice for 4 weeks.
RESULTSHuman fetal marrow MSCs were successfully cultivated and differentiated in vivo into many kinds of tissues such as bone, cartilage, adipose, skeletal muscle and tendon-like tissue.
CONCLUSIONHuman fetal marrow MSCs were multipotent stem cells.