The relationship between donor TNFalpha - 308 (G/A) genotype and recipient acute GVHD in allo-BMT.
- Author:
Jingbo WANG
1
;
Kaifeng PAN
;
Dan LI
;
Daopei LU
Author Information
- Publication Type:Journal Article
- MeSH: Acute Disease; Base Sequence; Blood Donors; Chromatography, High Pressure Liquid; methods; DNA; chemistry; genetics; Enzyme-Linked Immunosorbent Assay; Gene Frequency; Genotype; Graft vs Host Disease; blood; genetics; Hematopoietic Stem Cell Transplantation; Humans; Molecular Sequence Data; Sequence Analysis, DNA; Tumor Necrosis Factor-alpha; genetics; metabolism
- From: Chinese Journal of Hematology 2002;23(8):397-399
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo study the relationship between donor TNFalpha - 308 (G/A) genotype and recipient acute graft versus host disease (aGVHD).
METHODSTNFalpha - 308 (G/A) and TNFalpha - 308 (G/G) genotypes were analyzed by DHPLC and DNA sequence in twenty-one degree III/IV (III/IV) aGVHD patients, and twenty-eight degree 0/I (0/I) aGVHD patients. Serum TNFalpha levels were determined by ELISA.
RESULTSThe frequency of TNFalpha - 308 (G/A) genotype was significantly higher in III/IV aGVHD patients than in 0/I aGVHD patients (8/21 vs 1/28) (P < 0.05); and so did for higher serum TNFalpha levels (P < 0.05). TNFalpha levels were higher in III/IV aGVHD patients than in 0/I aGVHD patients in TNFalpha - 308 (G/G) patients (P < 0.01).
CONCLUSIONTNFalpha - 308 (G/A) is positively related to serum TNFalpha levels and most likely contributes to high risk for III/IV aGVHD.
