Promotion of post BMT hematopoiesis reconstitution by cotransplantation of IL-3 transfected marrow stromal cells in mice.
- Author:
Jiyang JIANG
1
;
Jingmei ZHANG
;
Jie HAO
;
Shusheng XIE
Author Information
- Publication Type:Journal Article
- MeSH: Animals; Bone Marrow Transplantation; Erythrocytes; cytology; Female; Hematopoiesis; genetics; physiology; Interleukin-3; genetics; Leukocytes; cytology; Mice; Mice, Inbred BALB C; Mice, Inbred C57BL; Stromal Cells; metabolism; transplantation; Time Factors; Transfection
- From: Chinese Journal of Hematology 2002;23(8):407-410
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo explore whether bone marrow stromal cell line QXMSC1 (H-2(d)) engineered to secrete IL-3 (QXMSC1 IL-3) can improve the hematopoiesis post-allogeneic bone marrow transplantation (BMT) in mice.
METHODSThe stromal cell line QXMSC1 IL-3 was established by transfecting QXMSC1 (H-2(d)) cell with a recombined retrovirus vector PL3SN containing mice IL-3 gene cDNA. Lethally irradiated mice C57BL/6 (H-2(b)) were transplanted with T cell depleted allogeneic bone marrow (BALB/c, H-2(d), 1 x 10(7)/mice) and QXMSC1 IL-3 cells (5 x 10(5)/mice). The numbers of RBC and WBC in peripheral blood were counted 20 and 40 days after bone marrow transplantation. The marrow nucleated cells, CFU-S, CFU-GM, CFU-E and CFU-GEMM yields were measured in recipient mice.
RESULTQXMSC1 IL-3 cells could stably secrete IL-3 and increase the peripheral RBC and WBC counts as well as the number of marrow nucleated cells and CFU-GM, CFU-E, CFU-GEMM yields.
CONCLUSIONCotransplantation of QXMSC1 IL-3 cells with T cell depleted marrow grafts improve hematopoiesis post allogeneic BMT in mice.
