Expression of B7 costimulator in eight human malignant hematological cell lines and its significance.
- Author:
Liping JIA
1
;
Xiaoyan KE
;
Jing WANG
Author Information
- Publication Type:Journal Article
- MeSH: B7-1 Antigen; Cell Line; Flow Cytometry; Humans; Interleukin-2; T-Lymphocytes; immunology; Transfection; U937 Cells
- From: Chinese Journal of Hematology 2002;23(7):345-348
- CountryChina
- Language:English
-
Abstract:
OBJECTIVETo explore the expression of B7 co-stimulator in human malignant hematological cell lines and its significance.
METHODSAmplified and purified plasmid DNA was transfected by liposome and detection of B7 gene expression by flow cytometry and RT-PCR.
RESULTEight human malignant hematological cell lines U937, K562, CEM, Daudi, GM, PEER, Jurkat, Raji express high level of HLA molecules, low or undetectable level of B7-1 gene, and high level of B7-2 gene. Expression level of B7-1 gene was enhanced after B7-1 transfection. T cell expressed high level of IL-2 mRNA after B7-1 transfection.
CONCLUSIONMultiple human malignant hematological cell lines express low or undetectable level of B7-1 gene, suggesting that B7-1 plays a critical role in tumor immunity.
