WT1 downregulation during K562 cell differentiation and apoptosis induced by bufalin.
- Author:
Yunpeng LIU
1
;
Xiujuan QU
;
Pingping WANG
;
Xin TIAN
;
Ying LUO
;
Shizhou LIU
;
Xianglan LU
Author Information
- Publication Type:Journal Article
- MeSH: Apoptosis; Cell Differentiation; Cell Proliferation; Down-Regulation; Humans; WT1 Proteins; genetics
- From: Chinese Journal of Hematology 2002;23(7):356-359
- CountryChina
- Language:English
-
Abstract:
OBJECTIVETo study the change of WT1 gene expression during human leukemic K562 cell differentiation and apoptosis induced by bufalin.
METHODSCell viability was determined by trypan blue exclusion, cell differentiation and apoptosis by nitro blue tetrazolium (NBT) reduction test, morphology and flow cytometry, expression of WT1 protein by Western blot analysis, and expression of WT1 mRNA by reverse transcriptase-polymerase chain reaction (RT-PCR).
RESULTS(1) The cell proliferation was inhibited by bufalin and the IC(50) at 24, 48, 72 h were 0.026, 0.032 and 0.006 micro mol/L, respectively. (2) Bufalin induced K562 cell differentiation towards macrophage/monocyte within concentration from 0.01 to 0.05 micro mol/L and apoptosis at higher than 0.026 micro mol/L. (3) The expression of WT1 protein and mRNA were downregulated by bufalin in the initial stage of differentiation and apoptosis induced by bufalin.
CONCLUSIONK562 cell differentiation and apoptosis induced by bufalin might relate to the downregulation of WT1 expression.
