Effects of As(2)O(3) on BCR-ABL protein level and signal transduction in CML cells.
- Author:
Zhongbin LAI
1
;
Guanlin SUN
;
Weili WU
;
Yingli WU
Author Information
- Publication Type:Journal Article
- MeSH: Fusion Proteins, bcr-abl; genetics; Humans; K562 Cells; Leukemia, Myelogenous, Chronic, BCR-ABL Positive; genetics; Phosphorylation; drug effects; Protein-Tyrosine Kinases; metabolism; Signal Transduction
- From: Chinese Journal of Hematology 2002;23(7):360-362
- CountryChina
- Language:English
-
Abstract:
OBJECTIVETo explore the effects of As(2)O(3) on BCR-ABL protein level and signal transduction in chronic myeloid leukemia (CML) cells.
METHODSImmunoprecipitation, protein tyrosine kinase (PTK) activity assay, real-time Taqman quantitative PCR and Western blot were used.
RESULTSAs(2)O(3) downregulated BCR-ABL protein and STAT1 protein of CML mononuclear cells in the concentrations of 1.0 approximately 2.0 micro mol/L and 0.5 approximately 2.0 micro mol/L after 48 h exposure, respectively. However, p27 protein level was not affected. The PTK activity of BCR-ABL protein was also mildly decreased in CML monouclear cells at 60 h. The bcr-abl mRNA level remained unchanged.
CONCLUSIONAs(2)O(3) downregulats BCR-ABL protein, STAT1 protein, BCR-ABL signal transduction and PTK activity in CML cells.
