Tumor antigen peptides from HL-60 cells induce specific immune response.
- Author:
Bo HUANG
1
;
Zuohua FENG
;
Guimei ZHANG
Author Information
- Publication Type:Journal Article
- MeSH: Cell Division; HL-60 Cells; HSP70 Heat-Shock Proteins; immunology; Humans; K562 Cells; Killer Cells, Natural; immunology; Leukocytes, Mononuclear; cytology; immunology; Neoplasm Proteins; immunology; Peptides; immunology
- From: Chinese Journal of Hematology 2002;23(6):285-288
- CountryChina
- Language:English
-
Abstract:
OBJECTIVETo prepare tumor antigen peptides from HL-60 cells and to induce specific immune response.
METHODSHL-60 antigen peptides were obtained using techniques including freezing and thawing, heat precipitation and acid precipitation. The stimulating effect of the in vitro Hsp70 binding HL-60 peptides on PBMC and the proliferation of stimulated PBMC were observed by T cell activation test. The cytotoxicity of proliferated PBMC is detected by incubating HL-60 cells or K562 cells with PBMC respectively.
RESULTSThe obtained tumor antigen peptides were a peptides mixture. The mixed peptides could activate PBMC and cause PBMC proliferation in vitro after presented by Hsp70. The proliferated PBMC showed specific cytotoxicity to HL-60 cells but not to K562 cells.
CONCLUSIONThe method for preparing of human leukemia tumor antigen peptides used in this paper is simple and easy; the obtained antigen peptides can induce specific immune response in vitro.
