Study of lymphocyte subsets and its activated-molecules in patients with paroxysmal nocturnal hemoglobinuria.
- Author:
Yuzhou WANG
1
;
Yongji WU
;
Zhinan ZHANG
Author Information
- Publication Type:Journal Article
- MeSH: ADP-ribosyl Cyclase; analysis; ADP-ribosyl Cyclase 1; Adult; Aged; Antigens, CD; analysis; CD28 Antigens; analysis; CD4 Antigens; analysis; CD4-Positive T-Lymphocytes; cytology; immunology; CD8 Antigens; analysis; CD8-Positive T-Lymphocytes; cytology; immunology; Female; Hemoglobinuria, Paroxysmal; blood; immunology; Humans; Killer Cells, Natural; cytology; immunology; Lymphocyte Subsets; cytology; immunology; Male; Membrane Glycoproteins; Middle Aged; Receptors, IgG; analysis
- From: Chinese Journal of Hematology 2002;23(11):571-573
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo investigate the relationship between subsets of lymphocytes and between its activated status and the clinical manifestations in patients with PNH, and to unfold immunological mechanism in the pathogenesis of PNH.
METHODSThe peripheral blood mononuclear cells (PBMNC) from 18 PNH patients and 20 controls were separated into two subpopulations using anti-CD(59) monoclonal antibody combined with goat-anti-mouse IgG immunomagnetic beads. CD(3)(+), CD(4)(+) and CD(8)(+) lymphocyte subsets were detected by flow cytometry. In 6 newly diagnosed patients, phenotypes associated with T cell activation such as CD(28)(+)/CD(4)(+) or CD(8)(+) cells, CD(8)(+) CD(38)(+) cells, and HLA-DR(+)/CD(4)(+) or CD(8)(+), and NK (CD(3)(-) CD(16)(+)) cells were detected in the peripheral blood.
RESULTPatients with PNH showed significantly increased CD(3)(+) CD(8)(+)/CD(3)(+) CD(4)(+) ratio as compared with controls (1.22 +/- 0.51 vs 0.86 +/- 0.27, P < 0.05), and the CD(3)(+) CD(8)(+)/CD(3)(+) CD(4)(+) ratio in CD(59)(-) PBMC was higher than that in CD(59)(+) PBMC (2.31 +/- 1.56 vs 0.62 +/- 0.27, P < 0.05). The ratios of CD(4)(+) CD(28)(+)/CD(4)(+) markedly decreased and CD(8)(+)HLA-DR(+)/CD(8)(+) increased.
CONCLUSIONPatients with PNH appear to have abnormalities in their lymphocytes. Increased ratios of CD(3)(+) CD(8)(+)/CD(3)(+) CD(4)(+) and HLA-DR(+) CD(8)(+)/CD(8)(+) lymphocytes as well as declined ratio of CD(4)(+) CD(28)(+)/CD(4)(+) lymphocytes might be involved in the pathogenesis of PNH.
