Expression of cell adhesion molecules in acute leukemia cell.
- Author:
Xiaoping JU
1
;
Min PENG
;
Xiaoping XU
;
Shuqing LU
;
Yao LI
;
Kang YING
;
Yi XIE
;
Yumin MAO
;
Fang XIA
Author Information
- Publication Type:Journal Article
- MeSH: Acute Disease; Adolescent; Adult; Aged; Bone Marrow Cells; metabolism; pathology; Cell Adhesion Molecules; genetics; Female; Gene Expression Regulation, Neoplastic; Humans; Intercellular Adhesion Molecule-1; genetics; Leukemia, Myeloid; genetics; pathology; Male; Middle Aged; Neural Cell Adhesion Molecules; genetics; Oligonucleotide Array Sequence Analysis; Precursor Cell Lymphoblastic Leukemia-Lymphoma; genetics; pathology; RNA, Messenger; genetics; metabolism; Reverse Transcriptase Polymerase Chain Reaction; Vascular Cell Adhesion Molecule-1; genetics
- From: Chinese Journal of Hematology 2002;23(11):581-584
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo investigate the role of cell adhesion molecule in the development and extramedullary infiltration (EI) of acute leukemia.
METHODSThe expressions of neural cell adhesion molecule (NCAM) gene, intercellular adhesion molecule-1 (ICAM-1) and vascular cell adhesion molecule (VCAM-1) genes in 25 acute leukemia patients bone marrow cells were detected by microarray and reverse transcriptase-polymerase chain reaction (RT-PCR).
RESULTSThe expressions of NCAM, ICAM-1 and VCAM-1 gene were significantly higher in acute leukemia cells and leukemia cells with EI than in normal tissues and leukemia cells without EI, respectively, both by cDNA microarray and by RT-PCR.
CONCLUSIONThe cDNA microarray is a powerful technique in analysis of acute leukemia cells associated genes. High expressions of cell adhesion molecule genes might be correlated with leukemia pathogenesis and infiltration of acute leukemia cell.
