In vitro expression of human factor VIII gene induced by sodium butyrate.
- Author:
Jun YIN
1
;
Hongli WANG
;
Xuefeng WANG
;
Haiyan CHU
;
Dao LI
;
Hongbing CHEN
;
Qihua FU
;
Baohua DUAN
;
Wenying KANG
;
Qiulan DING
;
Zhengwu QI
;
Zhenyi WANG
Author Information
- Publication Type:Journal Article
- MeSH: 3T3 Cells; Animals; Butyrates; pharmacology; Factor VIII; genetics; Gene Expression Regulation; drug effects; Humans; Mice
- From: Chinese Journal of Hematology 2002;23(9):463-465
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo explore the effect and mechanism of sodium butyrate on expression of human clotting factor VIII in vitro.
METHODSMouse NIH/3T3 cell line was transfected with recombinant plasmid vector pRC/RSV-BDD-hFVIII, which enclosed B-domain deleted (760aa approximately 1 639aa) human factor VIII cDNA (BDD-hFVIII cDNA). Then cells were incubated in Dulbecco's modification of Eagle's medium (DMEM) containing sodium butyrate for 24 hours, hFVIII: C and hFVIII: Ag in the cell culture medium were measured by ELISA assay and one-stage method, respectively. In addition, the effect of sodium butyrate on transcription of cDNA encoding the whole hFVIII, heavy and light chain of hFVIII was also investigated by means of run-on assay.
RESULTSAfter stimulation of sodium butyrate, the levels of hFVIII: C and hFVIII: Ag increased 70% than those of control. Run-on assay showed that sodium butyrate enhanced the transcription of cDNA which encoded heavy chain of hFVIII.
CONCLUSIONSodium butyrate can improve the expression of hFVIII through enhancing the transcription of hFVIII heavy chain encoding cDNA. It demonstrated that sodium butyrate had potential utility in inducing the expression of hFVIII in vitro.
