Effects of HLA disparity of two umbilical cord blood units on human engraftment in SCID mice.
- Author:
Liping ZHANG
1
;
Baijun SHEN
;
Huaishui HOU
;
Wenying YAN
;
Yunpeng DAI
;
Qing SHI
;
Xiufeng MA
;
Xiuli JU
;
Xingxia LIU
Author Information
- Publication Type:Journal Article
- MeSH: Animals; Antigens, CD; immunology; Cord Blood Stem Cell Transplantation; methods; Disease Models, Animal; Female; Fetal Blood; immunology; metabolism; Flow Cytometry; HLA Antigens; genetics; immunology; Hematopoiesis; Humans; Mice; Mice, SCID; Random Allocation; Severe Combined Immunodeficiency; immunology; physiopathology; surgery; Survival Analysis; Transplantation, Heterologous
- From: Chinese Journal of Hematology 2002;23(12):624-627
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo evaluate the feasibility and characteristics of human engraftment in HLA disparate cord blood transplantation.
METHODSTwo human HLA-haploidentical or HLA-mismatched cord blood units were transplanted into sublethally irradiated severe combined immunodeficiency (SCID) mice. The characteristics of engraftment, hematopoietic and immunological reconstitution between the two groups were compared.
RESULTSTwo mixed cord blood units can engraft in SCID mice with donor-recipient chimerism and reconstitute hematopoiesis and immunological functions. No unfavorable factors had been observed. Only one of the two cord blood units which had higher colony forming ability in vitro could engraft in most SCID mice as shown by HLA-DQB(1) gene detection. Two HLA-haploidentical cord blood units were simultaneously engrafted in 3 SCID mice.
CONCLUSIONDouble HLA-haploidentical or HLA-mismatched cord blood can engraft in SCID mice and reconstitute hematopoietic and immunological functions. HLA disparity has no significant effect on survival and engrafting rate. However, in less HLA disparity group, two cord blood units were prone to engraft simultaneously.
