Preparation and characterization of monoclonal antibody against human telomeric repeat binding factor 1.
- Author:
He HUANG
1
;
Jimin SHI
;
Qiaofang CHEN
;
Yi LUO
;
Wei DING
;
Jiyu LOU
Author Information
- Publication Type:Journal Article
- MeSH: Animals; Antibodies, Monoclonal; immunology; Blotting, Western; Female; Humans; Hybridomas; immunology; Immunohistochemistry; Mice; Mice, Inbred BALB C; Recombinant Fusion Proteins; immunology; Telomeric Repeat Binding Protein 1; analysis; genetics; immunology
- From: Chinese Journal of Hematology 2002;23(12):631-633
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo prepare a monoclonal antibody against human telomeric repeat binding factor 1 (TRF1) protein and explore its biological characteristics.
METHODSBALB/c mice were immunized with GST-TRF1(33-277) fusion protein for the preparation of monoclonal antibody by hybridoma technique. The obtained antibody was used for clinical assay by Western-blot and immunohistochemical staining.
RESULTSOne strain of hybridoma was obtained. It was confirmed by Western-blot that the antibody specifically recognized the 60 kD TRF1 protein. Immunohistochemical staining of the antibody showed that TRF1 protein located in the cytoplasm of epithelial cells and bone marrow cells.
CONCLUSIONA TRF1 monoclonal antibody, with high specificity was developed. It is useful for detection of TRF1 protein in tissue specimens.
