Arsenic trioxide induced p15INK4B gene expression in myelodysplastic syndrome cell line MUTZ-1.
- Author:
Hongyan TONG
1
;
Maofang LIN
Author Information
- Publication Type:Journal Article
- MeSH: Arsenicals; pharmacology; Cell Line; Cell Proliferation; drug effects; Cell Survival; drug effects; Cyclin-Dependent Kinase Inhibitor p15; genetics; DNA (Cytosine-5-)-Methyltransferase 1; DNA (Cytosine-5-)-Methyltransferases; genetics; DNA Methylation; drug effects; Gene Expression Regulation; drug effects; Humans; Myelodysplastic Syndromes; genetics; pathology; Oxides; pharmacology; RNA, Messenger; genetics; metabolism; Reverse Transcriptase Polymerase Chain Reaction
- From: Chinese Journal of Hematology 2002;23(12):638-641
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo investigate the mechanisms of arsenic trioxide (As(2)O(3)) induced demethylation.
METHODMethylation of p15INK4B gene in MUTZ-1 cell was detected by PCR using a methylation specific primer (MSP), the expression of P15, DNA methyltransferase (DNMT) 1, DNMT3A and DNMT3B gene by RT-PCR, the As(2)O(3) induced growth inhibition of MUTZ-1 cell by MTT method.
RESULTSP15 gene failed to express in MUTZ-1 cells after methylation. The expression was recovered after the cells exposed to As(2)O(3). As(2)O(3) could significantly down-regulate DNMT3A and DNMT3B but not DNMT1 gene on mRNA level in a dose dependent manner.
CONCLUSIONAs(2)O(3) could activate the expression of p15 gene by demethylation or/and by inhibiting DNMT3A and DNMT3B gene.
