Analysis and identification of transcriptional repression domain of ETO.
- Author:
Min WANG
1
;
Ling WANG
;
Chang-lai HAO
;
Hai-yan XING
;
Ke-jing TANG
;
Jian-xiang WANG
Author Information
- Publication Type:Journal Article
- MeSH: Core Binding Factor Alpha 2 Subunit; chemistry; genetics; Gene Expression Regulation, Leukemic; genetics; Genetic Vectors; Histone Deacetylases; physiology; Humans; In Vitro Techniques; Leukemia, Myeloid, Acute; enzymology; genetics; Oncogene Proteins, Fusion; chemistry; genetics; RUNX1 Translocation Partner 1 Protein; Transcription, Genetic; Transfection; Zinc Fingers; genetics
- From: Chinese Journal of Hematology 2003;24(1):10-13
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo further verify the transcriptional repression domains in ETO and their relationship with histone deacetylase (HDAC).
METHODSEither of the ETO two zinc fingers was mutated respectively by site-directing mutagenesis. The truncation fragments of ETO were amplified by polymerase chain reaction (PCR) and cloned into eukaryotic expression plasmid pFA-CMV. By the means of DNA transfection and analysis of the transcription derived from the promoter of reporter gene, the transcriptional regulation domains of ETO was determined.
RESULTSThe expression plasmids carrying truncated ETO and ETO with point mutation at either zinc finger were successfully constructed. Two repression domains were found within ETO, which were located at two zinc finger motifs and 275 - 487 amino acid residues, respectively.
CONCLUSIONThe transcription repression by ETO was mediated by two separated domains and closely associated with HDAC, which may be used as therapeutic target for acute myeloid leukemia M(2b).
