Identification of multidrug resistance related genes in leukemia by suppression subtractive hybridization.
- Author:
Ning-xi ZHU
1
;
Shu ZHENG
;
Rong-zhen XU
;
Rui-lan GAO
;
Jian-ping SHEN
;
Rong-xi YU
Author Information
- Publication Type:Journal Article
- MeSH: Blotting, Northern; Drug Resistance, Multiple; genetics; Drug Resistance, Neoplasm; genetics; Gene Library; Genes, MDR; genetics; Humans; K562 Cells; Leukemia; genetics; NADH Dehydrogenase; genetics; Nucleic Acid Hybridization; methods; Reverse Transcriptase Polymerase Chain Reaction; Ribosomal Proteins; genetics
- From: Chinese Journal of Hematology 2003;24(1):14-17
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo clone and screen genes related to multidrug resistance (MDR) in leukemia.
METHODSSuppression subtractive hybridization (SSH) was performed to profile differentially expressed genes between a MDR leukemia cell line (K562/DOX, as tester) and its parent cell line (K562, as driver). Reverse Northern dot blot was carried out to further screen the subtracted cDNA library. The overexpressed cDNA fragments in K562/DOX cells were sequenced and compared with known genes in Genbank. RT-PCR and Northern blot were employed to confirm the differential expression of some identified genes.
RESULTSEleven genes were identified being overexpressed in K562/DOX, including S3 ribosomal protein (S3rp) gene, NADH dehydrogenase subunit 2 (ND2) gene and My023 gene, which have not been reported to be related to MDR in cancer.
CONCLUSIONSeveral genes, which might be involved in MDR were identified, indicating novel mechanisms of MDR in leukemia.
