Effects of bcl-2 antisense oligodeoxynucleotide on proliferation and apoptosis of Raji cells.
- Author:
Fu-xu WANG
1
;
Zuo-ren DONG
;
Ze-lin LIU
;
Xue-jun ZHANG
;
Li YAO
;
Jing-ci YANG
;
Xing-yan DU
Author Information
- Publication Type:Journal Article
- MeSH: Apoptosis; drug effects; Cell Division; drug effects; Dose-Response Relationship, Drug; Flow Cytometry; Gene Expression Regulation, Neoplastic; drug effects; Humans; Oligonucleotides, Antisense; pharmacology; Proto-Oncogene Proteins c-bcl-2; genetics; metabolism; RNA, Messenger; drug effects; genetics; metabolism; Reverse Transcriptase Polymerase Chain Reaction; Tumor Cells, Cultured; drug effects; metabolism
- From: Chinese Journal of Hematology 2003;24(2):71-73
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo study the in vitro antitumor activity of bcl-2 fully phosporothioated antisense oligodeoxynucleotide (bcl-2 ASODN) to malignant lymphoblastic cells.
METHODSProliferation and apoptosis of Raji cells incubated with bcl-2 ASODN were evaluated by MTT assay, flow cytometry (FCM) and electron microscopy, and the level of bcl-2 protein and mRNA expression were assessed by FCM and RT-PCR, respectively.
RESULTSMTT assay demonstrated that bcl-2 ASODN could partially inhibit the growth of Raji cells. After incubated with ASODN for 48 hours, Raji cells exhibited characteristic morphologic changes of apoptosis, including cytoplasm membrane blebbing, chromatin condensation crescents formation and nuclear fragmentation. The apoptosis rate of Raji cells treated with 20 micromol/L bcl-2 ASON for 72 hrs was 43.86% which is significantly higher than that of control (10.05%). The bcl-2 ASODN induced apoptosis of Raji cells was accompanied by declined expression of bcl-2 mRNA, which decreased to 0.88% at 72 hrs and was significantly lower than that of control (79.54%).
CONCLUSIONbcl-2 ASODN induced Raji cells apoptosis by downregulating bcl-2 protein.
