Expression of gelatinase A and B in cord blood CD34+ cells and leukemic cell lines.
- Author:
Qing RAO
1
;
Yi-qi GENG
;
Li-li AN
;
Ke-fu WU
Author Information
- Publication Type:Journal Article
- MeSH: Antigens, CD34; analysis; Culture Media, Conditioned; metabolism; Electrophoresis, Polyacrylamide Gel; Fetal Blood; cytology; enzymology; immunology; HL-60 Cells; Humans; K562 Cells; Leukemia; enzymology; pathology; Matrix Metalloproteinase 2; biosynthesis; Matrix Metalloproteinase 9; biosynthesis; Tumor Cells, Cultured; U937 Cells
- From: Chinese Journal of Hematology 2003;24(2):78-81
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo study the expression of gelatinases, including matrix metalloproteinase-9 (MMP-9, gelatinase B) and matrix metalloproteinase-2 (MMP-2, gelatinase A), in CD(34)(+) cells and leukemic cell lines, and explore the significance of gelatinase in migrating and homing capacity of CD(34)(+) cells, as well as the role of gelatinase in leukemia pathogenesis.
METHODSCD(34)(+) cells were isolated from umbilical cord blood and normal bone marrow by Mini MACS system. By zymogram analysis, MMP-2 and MMP-9 were detected in the serum free condition medium of CD(34)(+) cells and cell lines.
RESULTSOne brilliant band with molecular weight of 92 x 10(3) was detected in condition medium of cord blood CD(34)(+) cells. No band was detected in condition medium of bone marrow CD(34)(+) cells. Brilliant bands with molecular weight of 92 x 10(3) and 72 x 10(3) were detected in the condition medium of U937, KG-1a and HL-60 cell lines, but not in that of HEL, Namalva, CEM, K562 and LCL-H cell lines. In the condition media of J6-1 and J6-2 cells only the 92 x 10(3) band was detected.
CONCLUSIONSCord blood CD(34)(+) cells produced MMP-9, but bone marrow CD(34)(+) cells did not, partly explains the fact that cord blood CD(34)(+) cells possessed higher migrating capacity in comparison with bone marrow CD(34)(+) cells. The expression of MMP-9 and MMP-2 in leukemic cell lines varied.
