Study on the relationship between hepatocellular carcinoma and the interaction between polymorphisms in DNA repair gene XPD and environmental factors
10.3760/cma.j.issn.0254-6450.2009.07.014
- VernacularTitle:DNA修复基因XPD单核苷酸多态性和环境因素的交互作用与肝细胞癌的关联研究
- Author:
Xiao-Yun ZENG
1
;
Xiao-Qiang QIU
;
Long JI
;
Hong-Ping YU
Author Information
1. 广西医科大学
- Keywords:
Hepatocellular carcinoma;
XPD gene;
Genetic polymorphisms;
Case-control study
- From:
Chinese Journal of Epidemiology
2009;30(7):702-705
- CountryChina
- Language:Chinese
-
Abstract:
Objective To study the relationship between hepatocellular carcinoma and the interaction of polymorphisms in DNA repair gene XPD with environmental factors. Methods A hospital-based ease-control study on hepatoeellular carcinoma was conducted. All the hepatocellular carcinoma eases (n=300) were newly diagnosed and controls (n=312) were diagnosed with non-tumor cases. XPD genotype (Lys751 Gin and Asp312 Ash) from blood derived DNA was determined using TaqMan MGB Real-time PCR. Unconditional logistic regression was used to estimate the odds ratios (ORs) and 95% confidence intervals (CIs). Results For XPD condon 751 genotypes, there was no significant difference between frequencies of the AC or CC among patients and controls (P>0.05) (referent AA). The frequency of XPD312A allelic gene was higher in eases than that in controls and was associated with an increased risk (adjusted OR = 2.62,95% CI: 1.626-4.222) for hepatocellular carcinoma when compared with GG genotype. Interactions were found between infection of HBsAg and XPD312 (OR=7.348), as well as between smoking and non-wild type gene of XPD751 (0R=4.291) and XPD312 (OR=5.341). Conclusion DNA repair XPD312A allelic gene might increase the risk of Hepatocellular carcinoma. Interactions between HBsAg infection, smoking and XPD were observed in Hepatocellular carcinoma.
