Genetic characterization of enterovirus 71 complete genome isolated in Beijing, 2008
10.3760/cma.j.issn.0254-6450.2009.07.022
- VernacularTitle:肠道病毒71型北京分离株全基因组序列分析
- Author:
Xin YAO
1
;
Qun-Ying MAO
;
Wei-Jin HUANG
;
Peng HE
;
Cheng ZHOU
;
Wei ZHANG
;
Zheng-Lun LIANG
;
Feng-Xiang LI
;
Jun-Zhi WANG
Author Information
1. 中国药品生物制品检定所
- Keywords:
Enterovirus 71;
Genotype;
Hand-foot-mouth disease
- From:
Chinese Journal of Epidemiology
2009;30(7):729-732
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the characterization of the complete genome of EV71 in Beijing, 2008 and to provide basis for selecting appropriate virus strain to develop vaccine. Methods 12 throat swab samples were collected from children with hand-foot-mouth disease (HFMD). One sample named 08YM-3 was cultured and isolated in vero cells. Viral RNA was extracted and carried out by RT-PCR and 5' , 3' rapid amplification of eDNA ends (RACE) to obtain the sequence from 08YM-3. PCR products were cloned and analyzed. Nucleotide identity between sequences was calculated and sequence alignments were made to generate phylogenetic trees using MegAlign in DNAStar. Results 3 clones were constructed that covered EV71 complete genome. Data from sequences analysis showed that this viral strain named BJ08 shared 95.6%-96.7%, 88.3%-96.1%,78.1%-94.0%,90.8%-94.6%, 85.9%-94.1% and 90.9%-93.9% in 5' UTR, PI, P2, P3, 3' UTR region and complete genome with C4 subtype, respectively. B J08 showed low nucleotides identity (<90%) with other subtypes. Phylogenetic trees established from alignment of the complete genome and VPI region indicated that B J08 belonged to C4 subtype. BJ08 and C4 subtype strains shared the same amino acids in 6 sites in VP1 region, which were associated with EV71 subtype. There was no mutation in VP1 antigen epitope (92-107aa). Conclusion This BJ08 strain belonged to C4 subtype. Further study on EV71 complete genome would have great significance for vaccine research.
